Gel Permeation Chromatography(GPC)

 | Post date: 2021/01/9 | 


Device model: waters GPC 2000
Manufacturer: Perkin Elmer
Country of manufacture: USA
Name of software used for analysis: Breeze

Description of test

Permeable gel chromatography (GPC) is a desirable analytical tool for the identification of natural and synthetic polymers and proteins that uses porous gel particles to separate polymers in solution. This technique separates molecules based on molecular size. A solution containing the desired polymer is pumped into a column containing porous particles. Due to the penetration, the polymer enters the cavities but only the polymers that are small enough enter the cavities and remain inside the cavities until they return to the flowing solvent. Smaller molecules take longer to pass through the gel pores and are delayed than larger molecules that spend less time in the pores. Therefore, the volume of extraction (extraction time) is proportional to the molecular size. Molecular weight is obtained relative by using a calibration curve and comparing the volume or time of extraction. Rapid advances in industry, medicine, pharmacy, nutrition technology, paints, adhesives, and synthetic fibers have led to an increase in the capabilities of GPCs. This device can operate in both cold and hot modes. Depending on the type of polymer and its solubility temperature in the desired solvent, cold or hot state is used.
Common solvents are THF (tetrahydrofuran) and toluene.

Services available:

Determination of molecular mass distribution

View: 970 Time(s)   |   Print: 115 Time(s)   |   Email: 0 Time(s)   |   0 Comment(s)